CHM
028 Organic Chemistry Laboratory II
Spring 2002
Dr. L. Zaikowski
Office: SCI 113 Phone: (631) 244-3180
Required
Text:
Introduction to Organic Laboratory Techniques
by Pavia, Lampman, and Kriz
Course
Objectives:
This
course applies the basic organic chemistry laboratory techniques that were developed
in the first semester to more challenging problems in synthesis and characterization.
The relevance of Organic Chemistry to daily life, and its ubiquitous nature
is presented through interesting essays and lectures. Analytical instrumentation
for the identification of compounds is further developed, and students obtain
experience with polarimetry, refractometry, HPLC, UV, IR, GC, GC/MS, and NMR
spectrometry. Students will improve their ability to write lab reports and maintain
a clear and well-organized lab notebook.
Requirements:
1. Complete reading assignments prior to class.
2. Write
a pre-lab prior to class in the lab notebook which should include:
a.) Title of expt., name of student, date of expt.
b.) Summary of Experiment and Essay readings
c.) Purpose
d.) Procedure and relevant equations, formula, chemical structures and data
3. In the
Lab:
a.) Wear safety goggles at all times in the lab. (Contact lenses are
not allowed in lab)
b.) Accurately record data and observations and provide an analysis of the data.
c.) Hand in carbon copies of the lab writeup at the end of the period.
d.) Clean up
Grading
Procedure
The
total lab points are summed and converted to percent to obtain the final grade.
In addition to lab reports, each lab period will count 10 points to reflect
laboratory technique and safety, including preparation and clean up.
CHM 028 Prof. Zaikowski Spring 2002
| Lab Period | Experiment | Reading Assignments |
| 1. Feb. 4 | Check In, clean glassware Lecture on Sulfa Drug Synthesis, exptl. set-up Assignment of SQUALOR Unknowns |
Laboratory SafetyReview Appendices 3, 4, 5 (IR/NMR) |
| 2. Feb. 11 | EXPT 42 (old textbook version) Synthesis of p-Acetamidobenzenesulfonylchloride and the Sulfa Drugs Sulfanilamide, Sulfapyridine, and Sulfathiazole |
Essay: Sulfa Drugs (p. 375) Techniques: vacuum filtration, reflux, gravity filtration, recrystallization |
| 3. Feb. 18 | EXPT 42 continued: Complete synthesis | |
| 4. Feb. 25 | EXPT 42 continued Mass determination, melting point, potassium bromide infrared spectroscopy Testing of Sulfa Drugs on Enterobacter aerogenes and Bacillus subtilis and PABA as an inhibitor |
Techniques: Melting point, KBr pellet, Infrared Spectroscopy Handout on Sterile Technique and Testing of Sulfa Drugs |
| 5. Mar. 4 | EXPT 42 continued: HPLC of Sulfanilamide, Sulfapyridine, and Sulfathiazole | (SQUALOR Unknowns due: 50 pts) HPLC handout/review Tech. 12 & 13 |
| 6. Mar. 11 | EXPT 59 ANALYSIS OF A DIET SOFT DRINK BY HPLC: Unknown determination by HPLC (handout) |
Essay: Local Anaesthetics (p. 388)Benzocaine Lab HPLC Protocol due (20 pts) (Expt 42 due: 100 pts) |
| 7. Mar. 18 | EXPT: Catalytic Conversion of Aldehydes (Handout) | Techniques: micro boiling point, reflux and distillation(HPLC Expt due: 30 pts) |
| 8. Apr. 1 | Catalytic Conversion of Aldehydes: GC/MS, IR, UV | Technique 15: Gas Chromatography |
| 9. Apr. 8 | Characterization of Aldehyde products | Technique 19: Prep. of Samples for Spectroscopy. Review NMRReview Mass Spectrometry(Ch. 12 McMurry) |
| 10. Apr. 15 | EXPT 54 LUMINOL: Synthesis and Luminescence | Essay: Fireflies/photochem. (p. 452) |
| 11. Apr. 22 | EXPT 58: Carbohydrates Pt I: Classification Tests | Ch. 25 in McMurry Lecture Text(Expt. 54 due: 30 pts) |
| 12. Apr. 29 | EXPT 58: Carbohydrates Part II: ID of Unknown | Carbohydrate lab due (50pts) |
| 13. May 6 | Check out/Make-up/BBQ/Sailing Excursion Plans | Aldehyde lab due (100pts) |